Pulmonary Medicine

Research Article

Factors Influencing Oxidative Imbalance in Pulmonary Fibrosis: An Immunohistochemical Study

Table 1

Source, concentrations, and pretreatment conditions of the antibodies used in the immunohistochemical assays. MWO: microwave oven.
NameSourceCloneWorking dilutionPretreatment
RAGEAbcam, Cambridge, UKGoat polyclonal1 : 4000none
MnSODStressgen, Victoria, CanadaRabbit polyclonal1 : 2000MWO 750W 15′
ECSODStressgen, Victoria, CanadaRabbit polyclonal1 : 400MWO 750W 15′
i-NOSNeoMarkers, Fremont, CA, USARabbit polyclonal1 : 200MWO 750W 15′
NitrotyrosinUpState, Billerica, MA, USARabbit polyclonal1 : 800MWO 750W 15′
NF-kBSanta Cruz Biotechnology, CA, USARabbit polyclonal1 : 450MWO 750W 15′
SMAD2Chemicon, Billerica, MA, USARabbit polyclonal1 : 2000MWO 750W 15′
SMAD3AbcamRabbit polyclonal1 : 100MWO 750W 15′