Perfluorochemical Liquid-Adenovirus Suspensions Enhance Gene Delivery to the Distal Lung
Comparison of luciferase activity in the lungs of mice. Mice were instilled with rAd-luc, and thoracic luciferase activity was quantified noninvasively over the course of 7 d. Two viral titers were administered viral particles (Low) or viral particles (High) in either saline or PFC liquid suspension. (a) viral particles delivered in saline; (b) viral particles delivered in PFC liquid; (c) viral particles delivered in saline; (d) viral particles delivered in PFC liquid. Each line represents the luciferase activity of a single animal. The images were acquired 10 min after intraperitoneal delivery of luciferin (150 mg/kg). Luminescent images were acquired under identical settings (3 min acquisition), and activity was assessed using an identical area (region of interest) and analyzed using the Living Image software package. The PFC liquid groups demonstrate a more consistent temporal pattern of expression compared to saline.