Long-Term Chronic Intermittent Hypobaric Hypoxia in Rats Causes an Imbalance in the Asymmetric Dimethylarginine/Nitric Oxide Pathway and ROS Activity: A Possible Synergistic Mechanism for Altitude Pulmonary Hypertension?

Figure 2

Comparison of (a) mRNA expression of eNOS by qRT-PCR and (b) sGC-dependent bioavailability of NO, expressed as the pVASP/VASP protein ratio. Representative bands on western blots are presented. The substrate availability of NOS, expressed as (c) mRNA expression of arginase isoform I determined by qRT-PCR, (d) mRNA expression of arginase isoform II determined by qRT-PCR, and (e) arginase activity (μmol urea/g protein/min) determined by spectrophotometry in the lungs of rats exposed to normoxia (NX), chronic intermittent hypoxia (CIH2x2), or chronic hypoxia (CH). Values are expressed as means and SE. ; ; ; in each group.

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