Rat Urinary Bladder Carcinogenesis by Dual-Acting PPAR Agonists
Figure 2
Lysis of the rat bladder urothelial cell layer in situ. (a), (b) On a fully
anesthetized rat, the bladder is exposed through an abdominal incision, a thin
needle or catheter (27G) is introduced into the bladder at the bladder neck,
and ligated in place with a silk suture. (c) The bladder is
emptied for urine, and filled with approximately 0.5 mL lysis solution (4 M
guanidine isothiocynate, 0.5% sarcosine, 25 mM citrate, pH 5.5), which is left
in place for 2 minutes and withdrawn. The resulting urothelial lysates can be
used for RNA isolation or protein analysis by Western, as described in [28]. By
infusing a trypsin solution into the bladder lumen, suspensions of urothelial
cells for flow cytometric analysis can be made [30].
