Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 1
The natural PPAR- agonist 15d- is a potent
inhibitor of microglial IL-12 p40 production in response to a vast array of TLR
ligands. Primary microglia were plated at cells/well in
96-well plates and incubated overnight. The following day, cells were
pretreated for 1 hour with the indicated concentrations of 15d- prior to the addition of TLR ligands. Cell-conditioned supernatants were
collected at 24 hours following TLR ligand exposure, whereupon IL-12 p40 levels
were determined by ELISA (a). The effects of 15d-PGJ2 on microglial
viability were assessed using an MTT assay (b). Significant differences between
microglia treated with TLR ligands alone versus TLR ligands + 15d- are noted with asterisks . Results presented are
representative of two independent experiments.
