Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 7
Pioglitazone augments microglial CXCL2 production in response
to Pam3Cys4 and ODN. Primary microglia were plated at cells/well in 96-well plates and incubated overnight. The following day, cells
were pretreated for 1 hour with the indicated concentrations of pioglitazone
prior to the addition of Pam3Cys4 or ODN. Cell-conditioned supernatants were
collected at 24 hours following TLR ligand exposure, whereupon CXCL2 levels
were determined by ELISA. Significant differences between microglia treated
with TLR ligands alone versus TLR ligands + pioglitazone are noted with
asterisks . Results presented are representative of two independent experiments.
