Microglia and Astrocyte Activation by Toll-Like Receptor Ligands: Modulation by PPAR- Agonists
Figure 8
15d-PGJ2 is a potent inhibitor of CCL2 release by
microglia in response to a wide range of TLR ligands. Primary microglia
were plated at cells/well in 96-well plates and incubated
overnight. The following day, cells were pretreated for 1 hour with the
indicated concentrations of 15d- prior to the addition of TLR
ligands. Cell-conditioned supernatants were collected at 24 hours following TLR
ligand exposure, whereupon CCL2 levels were determined by ELISA. Significant
differences between microglia treated with TLR ligands alone versus TLR ligands
+ 15d- are noted with asterisks . Results presented are
representative of two independent experiments.
