Activation of Penile Proadipogenic Peroxisome Proliferator-Activated Receptor with an Estrogen: Interaction with Estrogen Receptor Alpha during Postnatal Development
Figure 7
Micrograph sections from penile body of normal rat (a) and rats
treated neonatally with DES (b)–(e) or DES + ICI (f)-(g). Panel (a) was from a normal adult rat stained
with H and E for demonstration of normal histological structures of the penis
(a: dorsal artery; v: dorsal vein; CC: corpus cavernosum; CS: corpus
spongiosum; PU: penile urethra; TA: Tunica albuginea). Panels ((b), unstained) and ((c), stained for fat with 1% osmium tetroxide) were from a 28-day-old rat. Panels ((d), unstained) and ((e),
stained for fat with 1% osmium tetroxide and presented as a magnified view of
CC and CS regions) were from adult rat (120 days) treated neonatally with DES.
Note the empty appearing spaces of fat cells in CC regions in unstained
sections (panels (b) and (d)). In sections stained with 1% osmium tetroxide (to confirm presence of fat)
fat cells appear as black granules, *. Panels ((f),
unstained) and ((g), stained with 1%
osmium tetroxide) were from a 28-day-old rat treated neonatally with DES + ICI.
Note the absence of empty appearing fat cells and lack of fat staining in CC
region. Sections from these rats were used for immunolocalization of PPARγ in Figure 6
parts (a) & (b). Scale bars = 30 (E) and 200 m in other panels.
