Regulation of Translational Efficiency by Disparate -UTRs of PPAR Splice Variants
Figure 2
In vitro Transcription-Translation. Templates for the full-length PPAR-1, 2, 4, 5, and 7 splice variants were used to perform coupled in vitro transcription and translation reactions as described in “Methods”. Products were separated using SDS-PAGE. (a). The gel was dried and exposed to x-ray film to visualize in vitro labeled protein products. (b). (dotted bars) Band intensities were quantitated and the most optically dense band was set to 100. (b). (hatched bars) After the coupled in vitro transcription-translation reaction, labeled proteins were precipitated using TCA and the radioactivity in the precipitate was measured using a liquid scintillation counter. The highest counts were set to 100. (c). Templates for the 1, 2, 4, 5, and 7 splice variants were mixed with a pTRI-Xef template and in vitro transcription and translation reactions were performed on each mixture. Bands were resolved by SDS-PAGE. The bands for pTRI-Xef are shown with the band intensities reported below each band.
