The PPAR Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells
Figure 1
EP receptor subtypes and
PPAR are expressed in IMCD-K2 cells.
RNA
was isolated from IMCD-K2 cells using TRIzol, reverse transcribed, amplified by
polymerase chain reaction using primers for (a) the prostanoid receptors and (b) PPAR receptor, and visualized on an agarose gel using ethidium bromide under
ultraviolet light. As shown, specific bands for each PCR product were obtained at the
predicted size for (336 base pair), (437 base pair;
plus bands at 350 base pair and 600 base pair), and (423 base pair), as well as PPAR (161 base pair).
