The PPAR𝛿 Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells

Figure 10

Pretreatment with GW501516 does not significantly alter anisomycin-induced cleaved caspase-3 protein in IMCD-K2 cells. Protein was isolated from IMCD-K2 cells that had been treated with control serum-free media, 10⁻⁶ or 10⁻⁸ M GW501516, 250 ng/mL anisomycin (A250 or A) for 24 hours, 10⁻⁶ M GW501516 for 3 hours pretreatment followed by 10⁻⁶ M GW501516 for 24 hours with A250 (GW-6/A250), or 10⁻⁸ M GW501516 for 3 hours pretreatment followed by 10⁻⁸ M GW501516 for 24 hours with A250 (GW-8/A250). (a) The lysates were run on an SDS-PAGE gel and incubated with an anticleaved caspase-3 antibody (1:1000). (b) The membranes were stripped and incubated with anti-𝛽-actin antibody (1:10000) to normalize for densitometry. Expression is presented as fold A250. Values are mean ±  S.E.M.; 𝑛=8.

(a)

(b)