Research Article

The PPAR Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells

Figure 3

increases PPAR protein expression. Protein was isolated from IMCD-K2 cells that had been treated with serum-free media (control, C),  M indomethacin (Indo, I), or  M in the presence of I (PI) 2, 8, and 24 hours. The proteins were (a) run on an SDS-PAGE gel and Western blotted using an anti-PPAR antibody (1:500). A representative blot of 24-hour treatment is shown. The membranes were stripped and -actin was detected to normalize samples for (b) densitometric analysis. Expression is presented as fold of control. Values are means  S.E.M.; . compared to control; compared to indomethacin.
706283.fig.003a
(a)
706283.fig.003b
(b)