The PPAR Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells
Figure 3
increases PPAR protein expression. Protein was isolated from IMCD-K2
cells that had been treated with serum-free media (control, C), M indomethacin (Indo, I), or M in the presence of I (PI) 2, 8, and 24 hours. The proteins were (a) run on an SDS-PAGE gel and Western
blotted using an anti-PPAR antibody (1:500). A representative blot of 24-hour
treatment is shown. The membranes were stripped and -actin was detected to
normalize samples for (b)
densitometric analysis. Expression is presented as fold of control. Values are
means
S.E.M.; . compared to control; compared to indomethacin.