The PPAR𝛿 Ligand GW501516 Reduces Growth but Not Apoptosis in Mouse Inner Medullary Collecting Duct Cells

Figure 8

GW501516 has no effect on anisomycin-induced cell death. Serum-starved cells were stimulated with serum-free media (control), 10⁻⁶ M GW501516 (GW), 100 and 1000 ng/mL anisomycin (A100 and A1000, resp.), or 50 ng/mL cisplatin (Cis) for 18 hours. Some of the cells treated with anisomycin were pretreated with 10⁻⁶ GW501516 for 24 hours and cotreated with the agonist and anisomycin for 18 hours (GW/A100 and GW/A1000). The cells were then incubated for one hour with the DNA dye-binding solution from the CyQUANT NF assay kit (Invitrogen). Fluorescence intensity, representing number of cells, was then measured as per the manufacturer’s instructions. Values are means ±  S.E.M.; 𝑛=3. ^∗𝑃<.05 compared to control; §𝑃<.05 compared to GW.