Molecular Characterization of the Tumor Suppressor Candidate 5 Gene: Regulation by PPAR𝛾 and Identification of TUSC5 Coding Variants in Lean and Obese Humans

Figure 4

Experimental Overexpression of PPAR𝛾1 or PPAR𝛾2 in Mouse Livers in vivo Increases Adiponectin Expression but Fails to Strongly Increase Tusc5 mRNA Abundance. Mice were treated with adenoviruses containing LacZ (controls) or expression constructs to drive PPAR𝛾1 (left bars, 𝑛=3/group) or PPAR𝛾2 (right bars, 𝑛=6 & 7/group in controls and PPAR𝛾2, resp.). Either condition significantly increased PPAR𝛾 mRNA levels and adiponectin (AdipoQ) expression relative to LacZ controls (**𝑃<.01; ***𝑃<.001). Effects on Tusc5 expression were either not significant (PPAR𝛾1) or modest (PPAR𝛾2; 𝑃<.01). Means ± SEM are depicted.