Research Article
Peroxisome Proliferator-Activated Receptor -β/δ, -γ Agonists and Resveratrol Modulate Hypoxia Induced Changes in Nuclear Receptor Activators of Muscle Oxidative Metabolism
Table 1
Gene, accession number, forward and reverse primer sequences, and calculated efficiencies used for real-time PCR analysis.
| Gene | Accession no. | Forward primer | Reverse primer | Annealing temperature | Efficiency |
| SIRT1 | NM_019812.2 | -ATATTCCACGGTGCTGAGGT | -TCCAAATCCAGATCCTCCAG | 59 | 96.2% | PGC-1α | NM_008904.2 | -AACGATGACCCTCCTCACAC | -GGGTCATTTGGTGACTCTGG | 59 | 99.7% | RXR-α | NM_011305.3 | -GTTGCTTGTTTGCAATGGTG | -TGAGGAATATGGCCCAGAAG | 59 | 96.3% | PPAR-α | NM_011144.6 | -AACCGGAACAAATGCCAGTA | -CCGAATCTTTCAGGTCGTGT | 59 | 94.0% | PPAR-δ/β | NM_011145.3 | -TAGAAGCCATCCAGGACACC | -CCGTCTTCTTTAGCCACTGC | 59 | 95.6% | PPAR-γ | NM_001127330.1 | -CCAACTTCGGAATCAGCTCT | -CAACCATTGGGTCAGCTCTT | 59 | 95.1% | CPT-1b | NM_009948.2 | -CCCATGTGCTCCTACCAGAT | -CCTTGAAGAAGCGACCTTTG | 59 | 95.1% | UCP-2 | NM_011671.4 | -GCCTCTGGAAAGGGACTTCT | -AGAAGTGAAGTGGCAAGGGA | 59 | 97.7% | LOX | NM_010728.2 | -TGCTTGATGCCAACACCCA | -ATGCAAATCGCCTGTGGTAGC | 59 | 99.1% | RL7 | NM_011291.5 | -GGAGCTCATCTATGAGAAGGC | -AAGACGAAGGAGCTGCAGAAC | 59 | 99.1% |
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SIRT1, PGC-1α, RXRα, PPAR-α, -β, -γ, CPT-1b, UCP-2, LOX = lysyl oxidase, and RL7 = ribosomal protein L7.
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