Research Article

Peroxisome Proliferator-Activated Receptor -β/δ, -γ Agonists and Resveratrol Modulate Hypoxia Induced Changes in Nuclear Receptor Activators of Muscle Oxidative Metabolism

Table 1

Gene, accession number, forward and reverse primer sequences, and calculated efficiencies used for real-time PCR analysis.

Gene Accession no.Forward primerReverse primerAnnealing temperatureEfficiency

SIRT1NM_019812.2 -ATATTCCACGGTGCTGAGGT -TCCAAATCCAGATCCTCCAG59 96.2%
PGC-1αNM_008904.2 -AACGATGACCCTCCTCACAC -GGGTCATTTGGTGACTCTGG59 99.7%
RXR-αNM_011305.3 -GTTGCTTGTTTGCAATGGTG -TGAGGAATATGGCCCAGAAG59 96.3%
PPAR-αNM_011144.6 -AACCGGAACAAATGCCAGTA -CCGAATCTTTCAGGTCGTGT59 94.0%
PPAR-δ/βNM_011145.3 -TAGAAGCCATCCAGGACACC -CCGTCTTCTTTAGCCACTGC59 95.6%
PPAR-γNM_001127330.1 -CCAACTTCGGAATCAGCTCT -CAACCATTGGGTCAGCTCTT59 95.1%
CPT-1bNM_009948.2 -CCCATGTGCTCCTACCAGAT -CCTTGAAGAAGCGACCTTTG59 95.1%
UCP-2NM_011671.4 -GCCTCTGGAAAGGGACTTCT -AGAAGTGAAGTGGCAAGGGA59 97.7%
LOXNM_010728.2 -TGCTTGATGCCAACACCCA -ATGCAAATCGCCTGTGGTAGC59 99.1%
RL7NM_011291.5 -GGAGCTCATCTATGAGAAGGC -AAGACGAAGGAGCTGCAGAAC59 99.1%

SIRT1, PGC-1α, RXRα, PPAR-α, -β, -γ, CPT-1b, UCP-2, LOX = lysyl oxidase, and RL7 = ribosomal protein L7.