WY-14643, a Potent Peroxisome Proliferator Activator Receptor-α PPAR-α Agonist Ameliorates the Inflammatory Process Associated to Experimental Periodontitis
Figure 5
Effect of WY14643 on iNOS expression. Sections of gingivomucosal tissues from the contra lateral side obtained from vehicle-treated rats did not reveal any immunoreactivity for iNOS. (a) Positive staining for iNOS (b) was observed in gingivomucosal tissue after ligature. In gingivomucosal tissue of WY14643 treated rats no positive staining was observed for iNOS. (c) Densitometry analysis of immunocytochemistry photographs; ((d) photos from each samples collected from all rats in each experimental group) for iNOS from gingivomucosal tissue was assessed. The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Densitometry data are expressed as % of total tissue area. Moreover, a significant increase in iNOS expression (e, e1), assayed by Western blot analysis, was detected in in gingivomucosal tissue after ligature. Treatment with WY14643 significantly attenuated iNOS (e, e1) expression in gingivomucosal tissue after ligature. A representative blot of lysates (e, e1) obtained from 5 animals per group is shown and densitometry analysis of all animals is reported. Figure is representative of at least 3 experiments performed on different experimental days. versus nonligated. versus ligated.
