WY-14643, a Potent Peroxisome Proliferator Activator Receptor-α PPAR-α Agonist Ameliorates the Inflammatory Process Associated to Experimental Periodontitis
Figure 6
Effect of WY14643 on ligature-induced nitrotyrosine formation and PARP activation. No positive staining for nitrotyrosine was observed in gingivomucosal tissues from the contra lateral side obtained from vehicle-treated rats. (a) Positive staining for nitrotyrosine (b) was observed in gingivomucosal tissue after ligature. In gingivomucosal tissue of WY14643 treated rats no positive nitrotyrosine staining was observed. (c) In addition, immunohistochemistry for PAR, an indicator of in vivo PARP activation, revealed the occurrence of positive staining for PAR localized in gingivomucosal tissue after ligature. (f) WY14643 treatment reduced the degree of positive staining for PAR (g) in the gingivomucosal tissue. No positive staining for PAR was observed in gingivomucosal tissues from the contra lateral side obtained from vehicle-treated rats (e). Densitometry analysis of immunocytochemistry photographs ((d, h); photos from each samples collected from all rats in each experimental group) for nitrotyrosine and PAR from gingivomucosal tissue was assessed. The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Densitometry data are expressed as % of total tissue area. Figure is representative of at least 3 experiments performed on different experimental days. versus nonligated. versus ligated.
