Involvement of Fatty Acid Binding Protein 5 and PPAR/ in Prostate Cancer Cell Growth
Figure 2
Activation of PPAR results in upregulation of FABP5. (a) PC3M cells were treated with GW0742 (1 M, 4 h). Expression of FABP5, PDK1, ADRP, and VEGF were assessed by Q-PCR. The data were normalized to the untreated control. (b, c) Denoted cells were treated with 1 M (a) or 2 M (b) GW0742 for 16 h. Left panels, immunoblots of FABP5 in untreated versus GW0742-treated cells. Right panel: Densitometry analyses depicting changes in FABP5 expression upon treatment with GW0742 in three independent experiments (meanS.D.). (d, e) PC3M cells were transfected with vectors harboring SiScramble or PPAR siRNA. Three days later, expression of FABP5, PPAR, and VEGF mRNA in were measured by Q-PCR (d) and FABP5 expression was assessed by immunoblot (e). (f) PC3M cells were stably transfected with an empty vector (PGIPZ), or a vector harbouring shFABP5. Expression levels of FABP5, PPAR, and VEGF mRNA were measured by Q-PCR. * versus nontreated controls.
