TY - JOUR A2 - Ko, Joshua K. AU - Knoch, Bianca AU - Barnett, Matthew P. G. AU - Cooney, Janine AU - McNabb, Warren C. AU - Barraclough, Diane AU - Laing, William AU - Zhu, Shuotun AU - Park, Zaneta A. AU - MacLean, Paul AU - Knowles, Scott O. AU - Roy, Nicole C. PY - 2010 DA - 2010/06/30 TI - Molecular Characterization of the Onset and Progression of Colitis in Inoculated Interleukin-10 Gene-Deficient Mice: A Role for PPAR SP - 621069 VL - 2010 AB - The interleukin-10 gene-deficient (Il10-/-) mouse is a model of human inflammatory bowel disease and Ppara has been identified as one of the key genes involved in regulation of colitis in the bacterially inoculated Il10-/- model. The aims were to (1) characterize colitis onset and progression using a histopathological, transcriptomic, and proteomic approach and (2) investigate links between PPARα and IL10 using gene network analysis. Bacterial inoculation resulted in severe colitis in Il10-/- mice from 10 to 12 weeks of age. Innate and adaptive immune responses showed differences in gene expression relating to colitis severity. Actin cytoskeleton dynamics, innate immunity, and apoptosis-linked gene and protein expression data suggested a delayed remodeling process in 12-week-old Il10-/- mice. Gene expression changes in 12-week-old Il10-/- mice were related to PPARα signaling likely to control colitis, but how PPARα activation might regulate intestinal IL10 production remains to be determined. SN - 1687-4757 UR - https://doi.org/10.1155/2010/621069 DO - 10.1155/2010/621069 JF - PPAR Research PB - Hindawi Publishing Corporation KW - ER -