Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 2
Effects of selective PPAR agonists on TGF-1-induced collagens metabolism in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were exposed to Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M) for 2 h before stimulation with TGF-1 (10 ng/mL), in the presence or absence of PPAR agonists for 72 h. (a) Representative sections of Sirius red staining of beads (, G: 100X); (b) Representative map of Sirius red staining intensity (); (c) Mean overall quantification of Sirius red staining on beads sections (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as and from TGF-1-treated cells as .
