Activation of PPARs , /, and Impairs TGF-1-Induced Collagens' Production and Modulates the TIMP-1/MMPs Balance in Three-Dimensional Cultured Chondrocytes
Figure 3
Effects of selective PPAR agonists on TGF-1-induced type II collagen mRNA expression in rat chondrocytes. Chondrocytes, embedded in alginate beads (5.104 cells per bead), were exposed to Wy14643 (100 M), GW501516 (100 nM), or rosiglitazone (10 M) for 2 h before stimulation with TGF-1 (10 ng/mL), in the presence or absence of PPAR agonists for 48 h. Normalized mRNA levels of type II collagen were quantified by real-time PCR (). Data were expressed as mean ± SEM and statistically significant differences from the control were indicated as and from TGF-1-treated cells as .
