Peroxisome Proliferator-Activated Receptors Alpha, Beta, and Gamma mRNA and Protein Expression in Human Fetal Tissues

Figure 3

Lung. (a) The expression of PPAR$\alpha \text{,}$$\beta$, and $\gamma$ mRNA is shown across the fetal age range. Log plot of mean $\pm$ SEM Ct is normalized to $\beta$-2-microglobulin (B2M). (b) The fetal expression of PPAR$\alpha \text{,}$$\beta$, and $\gamma$ is shown relative to expression in adult lung. Each symbol represents the mean Ct value of 2 replicates for each fetal (open circles) sample and adult (filled circles) individual replicates are shown (overall mean for each group is shown as a horizontal line). (c) PPAR$\alpha \text{,}$$\beta$, and $\gamma$ protein expression is shown across the fetal age range. Western blot density normalized to glyceraldehyde-3-phophate dehydrogenase (GAPDH). Up arrowhead indicates PPAR$\alpha$, down arrowhead PPAR$\beta$, and diamond PPAR$\gamma$. If only one sample was available for a particular age, then an error term could not be calculated and no SEM bar is shown. Regression analysis evaluated change with age. Dashed lines in graphs of C are the 95% confidence interval.