Combination PPAR𝛾 and RXR Agonist Treatment in Melanoma Cells: Functional Importance of S100A2

Figure 1

S100A2 mRNA stimulation by TZD/rexinoid treatment is dependent on intact PPAR$\gamma$ and RXRγ. One microgram of total RNA was used for the S100A2 quantitative reverse transcription-PCR analysis (ABIPRISM7700; Perkin-Elmer), and absolute values were derived from a standard curve using a known amount of sense strand RNA (ag, attograms of sense strand RNA). Isoform RNA was normalized to total input RNA (18s rRNA measured from 1 ng of total RNA). A375(DRO) cells were infected with either shPPAR$\gamma$ or shRXRγ lenteviral particles and then treated with LGD1069/ROSI 1 μM for 24 hours. S100A2 mRNA levels were compared to levels from A375(DRO) cells infected with the shSCR control under the same treatment conditions.