Figure 4: Microarray and Real-time PCR analysis of selected genes. Data from both assays were in close agreement. Small changes in Ndufa5 expression, a gene which encodes for a subunit of mitochondrial respiratory chain complex I, could not be confirmed by RT-PCR. As predicted based on microarray analysis, PFOS did not appear to up-regulate the expression of Srebf2, Ppargc1a (Pgc-1a), or Nfe2l2 (Nrf2) in WT or Null mice. Red or green correspond to average up- or down- regulation, respectively.