Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet

Figure 6

Rosiglitazone reduced macrophage and pro-inflammatory markers by gene expression as well as ex vivo spontaneous mediator production. (a) Gene expression of macrophage markers was analyzed from the epididymal fat pads. Data is represented as DIO gene expression fold induction versus the chow cohort or DIO animals treated with Rosiglitazone versus the DIO cohort ($n=8$ mice/group). Statistical significance was determined by a two-tailed Welch t-test; *$P<.05$, **$P<.01$, and ***$P<.001$. (b) Stromal vascular cells were isolated from the EF and incubated ex vivo. Spontaneous mediator production was assessed ($n=\mathrm{\hspace{0.17em}\hspace{0.17em}}8$ mice/group). Values represent the mean and SEM. Mann-Whitney U test was used to compare standard chow to DIO or DIO to DIO/rosiglitazone; *$P<.05$ and *$P<.01$.

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