Kinetic Assessment and Therapeutic Modulation of Metabolic and Inflammatory Profiles in Mice on a High-Fat and Cholesterol Diet
Table 2
Elevated serum metabolic parameters and inflammatory mediators in the DIO cohort.
6-weeks
16-weeks
27-weeks
Parameter
CHOW
DIO
CHOW
DIO
CHOW
DIO
Insulin, pg/ml
57.8 (41)
383.4 (76)*
710.9 (145)
2901.0 (716)*
1458 (446)
2602.5 (289)*
ALT, U/ml
23.0 (1.1)
29.5 (2.0)*
29.1 (5.3)
174.2 (40.9)*
22.9 (1.9)
82.7 (11.6)*
Leptin, ng/ml
0.69 (0.30)
58.8 (14.6)*
7.1 (1.5)
32.9 (12.5)*
4.6 (1.2)
57.0 (12.6)*
CCL2, pg/ml
0
4.3 (1.1)
18.9 (2.4)
42.1 (3.4)*
168.6 (21)
234.7 (14.4)*
IL-6, pg/ml
0
3.5 (0.6)
9.6 (0.7)
25.5 (7.7)#
32.7 (11.2)
54.1 (17)
TNF, pg/ml
15.9 (2.1)
19.9 (2.3)
13.2 (0.4)
13.2 (0.5)
1.6 (0.7)
1.5 (0.3)
IL-1b, pg/ml
15.9 (2.1)
19.9 (2.3)
34.8 (1.4)
37.4 (1.5)
0.9 (0.2)
1.1 (0.2)
IL-10, pg/ml
21.8 (9.2)
33.1 (6.8)
39.1 (1.8)
66.8 (16.4)
22.1 (7.3)
69.8 (33)
At each timepoint, mice from separate groups of animals were terminally bled (Chow mice and the DIO mice). Values represent the mean and SEM. Mann-Whitney U test was used to compare Chow to DIO at each time point; * and #.
