Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver
Figure 4
Expression pattern of hepatic cholesterol-regulatory genes under HNF4α overexpression in rat liver. (a) Total RNA was extracted from rat liver and the mRNA levels of hepatic cholesterol-regulatory genes, including HMGCoAR, LCAT, PLTP, LDLR, SR-BI, ABCA1, ABCG5, ABCG8, and CYP7A1, were measured by qPCR. Data are mean ± SEM mRNA levels normalized to that of β-actin and expressed as fold of GFP-infected group. *. (b) Rat liver extracts were examined by western blot analysis with antibodies against SR-BI or β-actin.
