Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver

Figure 5

HNF4α-upregulated SR-BI depends on PPARγ. Rat primary hepatocytes were pretreated with or without PPARγ antagonist BADGE (10 μM) for 30 min and then infected with Ad-HNF4α or Ad-GFP. (a) Real-time PCR analysis of mRNA expression and (b) western blot analysis of protein expression from 3 independent experiments. β-actin was used as an internal control. Data are mean ± SEM mRNA levels normalized to that of β-actin and expressed as fold of control group. *𝑃<0.05, **𝑃<0.01.

(a)

(b)