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PPAR Research
Volume 2011, Article ID 179454, 11 pages
http://dx.doi.org/10.1155/2011/179454
Research Article

Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H/10T1/2 Adipocytes

1Metabolic Discovery Technology Group, Molecular Discovery Research, GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, NC 27709, USA
2Cheminformatics, Molecular Discovery Research, GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, NC 27709, USA
3Oncology Discovery Technology Group, Molecular Discovery Research, GlaxoSmithKline, 709 Swedeland Road, King of Prussia, PA 19406, USA
4Discovery Analytics, GlaxoSmithKline, 5 Moore Drive, Research Triangle Park, NC 27709, USA
5Integrative Biology, High Throughput Biology, Discovery Research, GlaxoSmithKline, 709 Swedeland Road, King of Prussia, PA 19406, USA

Received 29 April 2011; Revised 20 July 2011; Accepted 4 August 2011

Academic Editor: Beata Lecka-Czernik

Copyright © 2011 James X. Rong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure 1. Triglyceride content in differentiated C3H/10T1/2 adipocytes incubated in washout buffer.

Figure 2. Relative mRNA abundance of brown adipose tissue markers Ucp1 and Cidea in C3H/10T1/2 adipocytes and 3T3-L1 adipocytes, determined by QRT-PCR.

Figure 3. Adipsin (Adn), fatty acid synthase (Fasn), and glucose transporter 1 (Slc2a1), were regulated differently by RSG in 3T3-L1 and C3H/10T1/2 adipocytes.

Table 1. Regulation of adipocyte and fibroblast/preadipocyte marker genes with the washout conditions in C3H/10T1/2 cells (A), and 3T3 L1 cells (B), and responses of PDK4 and PCK1 to rosiglitazone in C3H/10T1/2 and 3T3-L1 adipocytes (C).

Table 2. QRT-PCR primer/probe sequences.

Table 3. Expression of brown adipose tissue markers in 3T3 L1 and C3H/10T1/2 adipocytes.

Table 4. Regulation of genes by RSG in 3T3-L1 (A) and C3H/10T1/2 (B) adipocytes over time.

Table 5. Confirmation of the microarray data using QRT-PCR.

Table 6. Presence of PPAR-responsive element consensus regions on the promoters of nucleus-encoded mitochondrial genes.

  1. Supplementary Materials