Consequences of PPAR_α Invalidation on Glutathione Synthesis: Interactions with Dietary Fatty Acids

Table 3

Hepatic thiols concentrations and pools in WT and PPARα-null (KO) mice fed diets containing either saturated FA (COCO diet) or ALA (LIN diet) for 8 weeks.

	WT		KO		𝑃 values
	COCO	LIN	COCO	LIN	ANOVA	Genotype; (G)	Diet; (D)	Interaction G∗D
GSH (μmol/g of protein)	3 112 ± 180^b	5 138 ± 412^a	5 201 ± 509^a	3 892 ± 382^ab	0.0013	0.2815	0.3569	0.0003
Cysteine (μmol/g of protein)	274 ± 91.8	190 ± 66.0	211 ± 55.8	243 ± 55.8	0.8377	0.9432	0.7061	0.4144
CysGly (μmol/g of protein)	97.5 ± 5.80	83.6 ± 12.7	102 ± 20.5	93.5 ± 8.14	0.8012	0.6243	0.4461	0.8514
GSH (μmol/ liver)	439 ± 31.3^b	774 ± 77.2^ac	1031 ± 118^a	544 ± 80.7^bc	0.0002	0.0367	0.3590	<.0001
Cysteine (μmol/ liver)	40.2 ± 16.0	29.3 ± 10.6	41.3 ± 10.1	33.2 ± 7.62	0.8614	0.8304	0.4181	0.9034
CysGly (μmol/ liver)	14.2 ± 1.24	13.1 ± 2.03	20.0 ± 3.49	12.9 ± 1.19	0.1456	0.2600	0.1099	0.2383

Values are means ± standard errors for 7 mice per group. Mean values within a row sharing a same superscript letter, or without superscript letter, were not significantly different at 𝑃<0.05.