Cardiomyocyte-Restricted Deletion of PPAR<i>β</i>/<i>δ</i> in PPAR<i>α</i>-Null Mice Causes Impaired Mitochondrial Biogenesis and Defense, but No Further Depression of Myocardial Fatty Acid Oxidation

<table>Endogenous anti oxidants and oxidative stress. (a) Real-time PCR analysis of transcript expression of SOD1, SOD2, and catalase on samples from TMCM, PPAR<i>α </i><sup>−/−</sup>, TMPD, and TMPDPA hearts. (b, c, and d) Western blotting analysis of protein levels of SOD1, SOD2, and catalase on samples from TMCM, PPAR<i>α </i><sup>−/−</sup>, TMPD, and TMPDPA hearts. (e) Mitochondrial membrane potential estimated by JC-1 uptake assay in isolated mitochondria from TMCM, PPAR<i>α </i><sup>−/−</sup>, TMPD, and TMPDPA hearts. (f) Mitochondrial aconitase activity of samples from TMCM, PPAR<i>α </i><sup>−/−</sup>, TMPD, and TMPDPA hearts. <svg height="15.4375" id="M8" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 65.099998 15.4375" width="65.099998" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.4375)">
<g transform="translate(72,-59.65)">
<text transform="matrix(1,0,0,-1,-69.87,67.83)">
<tspan style="font-size: 8.75px; " x="0" y="0">∗</tspan>
</text>
<text transform="matrix(1,0,0,-1,-64.79,61.87)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.9" y="-0.95999998">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.927885" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="29.179386" y="-0.95999998">.</tspan>
<tspan style="font-size: 12.50px; " x="32.305138" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="38.556637" y="-0.95999998">5</tspan>
</text>
</g>
</g>
</svg> versus TMCM; <svg height="15.4375" id="M9" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 70.8125 15.4375" width="70.8125" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,15.4375)">
<g transform="translate(72,-59.65)">
<text transform="matrix(1,0,0,-1,-69.87,67.83)">
<tspan style="font-size: 8.75px; " x="0" y="0">∗</tspan>
<tspan style="font-size: 8.75px; " x="4.5772958" y="0">∗</tspan>
</text>
<text transform="matrix(1,0,0,-1,-60.21,61.87)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
<tspan style="font-size: 12.50px; " x="10.9" y="-0.95999998">&lt;</tspan>
<tspan style="font-size: 12.50px; " x="22.927885" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="29.179386" y="-0.95999998">.</tspan>
<tspan style="font-size: 12.50px; " x="32.305138" y="-0.95999998">0</tspan>
<tspan style="font-size: 12.50px; " x="38.556637" y="-0.95999998">1</tspan>
</text>
</g>
</g>
</svg> versus TMCM.</table>

PPAR Research

fig5

Figure 5

Figure 5: Cardiomyocyte-Restricted Deletion of PPAR<i>β</i>/<i>δ</i> in PPAR<i>α</i>-Null Mice Causes Impaired Mitochondrial Biogenesis and Defense, but No Further Depression of Myocardial Fatty Acid Oxidation 

Figure 5 | Cardiomyocyte-Restricted Deletion of PPARβ/δ in PPARα-Null Mice Causes Impaired Mitochondrial Biogenesis and Defense, but No Further Depression of Myocardial Fatty Acid Oxidation 