Figure 4: Downregulation of PPARs by siRNA alters ω-6 lipid-mediated induction of Egr-1 mRNA: RASMCs were transfected with SMART-pool siRNA duplexes of PPARα, PPARγ or nontargeting control siRNA, followed by treatment with ω-6 lipids for 4 and 12 hrs. Preliminary findings indicated Egr-1 mRNA levels were upregulated when PPARα was knocked down compared to PPARγ knockdown as determined by qRT-PCR. The fold change was calculated by comparing lipid treatments with vehicle controls in each siRNA group. All values were normalized to β-actin (house-keeping gene). mRNA levels were expressed as fold change ± SEM (Standard error of ΔCT mean).