Figure 5: Alterations in Egr-1/MCP-1 by ω-6 lipids resulted in altered monocyte (THP1) adhesion to RASMCs THP1 (human monocyte) cells adhesion to RASMCs exposed to LA and OxLA (with or without pretreatment to MK886 and GW9662) was determined by counting the number of cells in a 3 × 3 field on an inverted fluorescent microscope. Results showed that monocyte adhesion reflected the alterations in Egr-1 and MCP-1 levels by ω-6 lipids by an increase in adherent monocyte cell number at 4 hrs when PPARα was downregulated and increase in adherent cell number at 12 hrs when PPARγ was downregulated. The data presented represents mean numbers of THP1 cells adhered to RASMCs in each field ± SEM (Standard Error of Mean). (a) monocyte adhesion at 4 hrs; (b) monocyte adhesion at 12 hrs. * 𝑃 < 0 . 0 5 , ** 𝑃 < 0 . 0 1 , *** 𝑃 < 0 . 0 0 5 .