Conditional Expression of Human PPARδ and a Dominant Negative Variant of hPPARδ In Vivo

Figure 3

Mice expressing functional hPPARδ and hPPARδΔAF2 protein. Animals (𝑛=5 per group, 12 weeks old, mixed males and females) were placed on a diet containing I3C (0.25% (w/w)) (open bars) or a diet containing I3C (0.25% (w/w)) and GW501516 (0.0025% (w/w)) (black bars) for 14 days. (a) body weight of nontransgenic (Non-Tg), hPPARδ and hPPARδΔAF2 transgenic mice expressed as a percentage of initial body weight. (b–f) mRNA expression of Acox1 in liver (b), muscle (c), mRNA expression of Adrp in liver (d), muscle (e), and mouse endogenous PPARδ (f) following treatments outlined above. Statistical significance (^∗𝑃≤0.05; ^∗∗𝑃≤0.01; ^∗∗∗𝑃≤0.001) where indicated was analysed using Mann-Whitney non-parametric test in GraphPad Prism version 5.0c, (GraphPad Software, San Diego, CA, USA).

(a)

(b)

(c)

(d)

(e)

(f)