Rosiglitazone Affects Nitric Oxide Synthases and Improves Renal Outcome in a Rat Model of Severe Ischemia/Reperfusion Injury
Figure 3
Effect of 5 mg/kg rosiglitazone (RGZ) on the mRNA levels of (a) endothelial and (b) inducible NO-Synthase (eNOS and iNOS) in Sprague-Dawley rats in renal I/R injury. Total RNA was generated from kidney cortex. Real-time PCR against eNOS, iNOS, and β-actin was performed as described in Section 2. Quantification was performed using the DDC T method using β-actin as a reference gene, and expression in untreated control animal (control 0 h) was normalized to 100. n is given in the respective bars. (a) Effect RGZ on the mRNA levels of eNOS. The amount of eNOS mRNA signal normalized to the respective signal from β-actin. #,* Statistically significant difference as indicated. n.s.: Statistically not significant. (b) Effect of RGZ on the mRNA levels of iNOS. The amount of iNOS mRNA signal normalized to the respective signal from β-actin. #,*Statistically significant.
