AS601245, an Anti-Inflammatory JNK Inhibitor, and Clofibrate Have a Synergistic Effect in Inducing Cell Responses and in Affecting the Gene Expression Profile in CaCo-2 Colon Cancer Cells
Figure 6
PPRE binding activity and Western Blot analysis of Med1 and cyclin D1. (a) PPRE binding activity of PPARα. Results represent the absorbance, detected at 450 nm, subtracted from the control value, and are the mean ± SD of three separate experiments from three different preparations for each condition; (b) Western Blot analysis of Med1 protein detected in CaCo-2 cells treated with 5 μM and 50 μM clofibrate (Clofi), 0.1 μM AS1245, and 5 μM clofibrate plus 0.1 μM AS601245 (Clofi + AS601245), collected at 24 hours after the treatment; (c) Western Blot analysis of Med1 protein detected in CaCo-2 cells treated with 5 μM clofibrate, 0.1 μM AS1245, and 5 μM clofibrate plus 0.1 μM AS601245 (Clofi + AS), collected at 24, 48, and 72 hours after the treatment; (d) Western Blot analysis of cyclin D1 detected in CaCo-2 cells treated with 5 μM and 50 μM clofibrate (Clofi), 0.1 μM AS1245, and 5 μM Clofibrate plus 0.1 μM AS601245 (Clofi + AS601245), collected at 24 hours after the treatment. Graphics represent the relative quantification of protein products performed by densitometric scanning. Data were normalized by using the β-actin signal, expressed as arbitrary densitometric units, and are the mean ± SD of three separate experiments from three different preparations for each condition. Variance analysis: *, ** versus control.
