Research Article

Peroxisome Proliferator-Activated Receptor Agonists Differentially Regulate Inhibitor of DNA Binding Expression in Rodents and Human Cells

Figure 2

Downregulation of Id2 gene expression by WY is dependent on PPARα. Wild-type SV129 mice (+/+) or SV129 mice that lack PPARα (−/−) were fed a control diet (Control) or a diet containing WY (0.1%) or DEHP (0.6%) for 3 weeks. Total RNA isolated from liver was separated by 1.2% agarose, transferred to nylon, and analysed by northern blot using probes for Id2, Ehhadh, and 17βHSD, and GAPDH as a control. Northern autoradiograms (a) were densitometrically scanned and expression normalized to that of GAPDH (b). Each value represents the mean ± standard error of three animals. *significantly different from control ( ).
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(a)
483536.fig.002b
(b)