Research Article

Peroxisome Proliferator-Activated Receptor Agonists Differentially Regulate Inhibitor of DNA Binding Expression in Rodents and Human Cells

Figure 3

Fenofibrate upregulates Id2 gene expression in HepG2 cells. Panel (a) human hepatocarcinoma cells treated with different concentrations of fenofibrate (0, 10, 50, or 100 μM) for 2, 6, or 24 hours. Relative Id2 mRNA levels were measured by real-time PCR, normalized to β-actin levels and expressed in relative units to control. Values for Id2 mRNA are expressed as mean ± SD ( ). Panel (b) HepG2 cells were preincubated with the PPARα antagonist MK-886 (10 μM) where indicated and treated with different concentrations of fenofibrate (0 or 50 μM) for 24 hours.
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