Fenofibrate Enhances the In Vitro Differentiation of Foxp3⁺ Regulatory T Cells in Mice

Figure 3

WY14643, gemfibrozil, and bezafibrate did not affect Treg differentiation in vitro. Total and CD4⁺ T cells were isolated from mouse spleens and induced to differentiate into Treg cells with 5 ng/mL TGF-β and 50 U/mL IL-2. 100 μM WY14643 (a), 50 μM gemfibrozil (b) and 30 μM bezafibrate (c) or solute control were added respectively. 4 days later the percentage of Foxp3⁺ T cells was analyzed with flow cytometry. The outlined areas indicate Foxp3⁺ T cells, and the percentages were shown in the lower right corner. Data represent 1 of at least 3 independent experiments.

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