Research Article

Cinchonine Prevents High-Fat-Diet-Induced Obesity through Downregulation of Adipogenesis and Adipose Inflammation

Table 1

Primer sequences and PCR conditions.

Gene descriptionPrimersSequences (5′→3)Annealing
temperature(°C)
PCR
product (bp)

Peroxisome proliferator-activated receptor gamma2 (PPARγ2)F
R
TTCGGAATCAGCTCTGTGGA
CCATTGGGTCAGCTCTTGTG
55148
CCAAT/enhancer-binding protein, alpha (C/EBPα)F
R
AAGGCCAAGAAGTCGGTGGA
CCATAGTGGAAGCCTGATGC
55189
Adipocyte protein 2 (aP2)F
R
ACATGAAAGTGGGAGTG
AAGTACTCTCTGACCGGATG
55128
LeptinF
R
CTCCAAGGTTGTCCAGGGTT
AAAACTCCCCACAGAATGGG
55143
Sterol regulatory element-binding factor 1 (SREBP1c)F
R
TTGTGGAGCTCAAAGACCTG
TGCAAGAAGCGGATGTAGTC
5594
Lipoprotein lipase (LPL)F
R
TGCCGCTTTTTGTTTTACC
TCACAGTTTCTGCTCCCAGC
55172
Secreted frizzled-related protein 5 (SFRP5)F
R
CTTGGTGTCCTTGCGCTTTA
CTGATGGCCTCATGGAACAG
61155
Wingless-type MMTV integration site family, member 10B (WNT10b)F
R
TTTTGGCCACTCCTCTTCCT
TCCTTTTCCAACCGAAAACC
61183
Dickkopf 2 (DKK2)F
R
GCATTTCCTTCAGATTGGCA
TCATTCCCTGTTCTTCAGCG
55144
Galanin receptor 1 (GalR1)F
R
CCAAGGGGGTATCCCAGTAA
GGCCAAACA CTACCAGCGTA
55147
Galanin receptor 2 (GalR2)F
R
ATAGTGGTGCTCATGCTGGAA
AGGCTGGATCGAGGGTTCTA
60134
Protein kinase C δ (PKCδ)F
R
CTGAGCGCTGCAAGAAGAAC
TGGAAACTTTGATCCTGCACTGA
60146
Cyclin D (Cyc-D)F
R
TGGGAAGTTTTGTTGGGTCA
TCCTTGTCCAGGTAATGCCA
55144
E2F1F
R
CCTGGAGCATGTTAAAGAAG
CCTCGAGACCAAAGTGATAG
48.9102
Toll-like receptor 2 (TLR2)F
R
TCTAAAGTCGATCCGCGACA
TACCCAGCTCGCTCACTACG
56.2344
Toll-like receptor 4 (TLR4)F
R
ACCTCTGCCTTCACTACAGA
AGGGACTTCTCAACCTTCTC
48.6223
Myeloid differentiation primary response gene 88 (MyD88)F
R
AAGAAAGTGAGTCTCCCCTC
TCCCATGAAACCTCTAACAC
55149
Toll-interleukin 1 receptor domain-containing adaptor protein (Tirap) F
R
GTGGCCGCTGGAGCAAAGAC
TTGCCTCTGCCATCCACATA
55370
TNF receptor-associated factor 6 (TRAF6)F
R
GCACAAGTGCCCAGTTGACA
AGTGTCGTGCCAAGTGATTC
62.7479
TIR-domain-containing adapter-inducing interferon-β (TRIF)F
R
ATGGATAACCCAGGGCCTT
TTCTGGTCACTGCAGGGGAT
56.5528
Interferon regulatory factor 5 (IFR5)F
R
AATACCCCACCACCTTTTGA
TTGAGATCCGGGTTTGAGAT
53.1191
Interferon α (IFNα)F
R
ATGGCTAG(G/A)CTCTGTGCTTTCCT
GGGCTCTCCAGA(T/C)TTCTGCTCTG
60.2500
Tumor necrosis factor alpha (TNFα)F
R
TGTCTCAGCCTCTTCTCATT
AGATGATCTGAGTGTGAGGG
55156
Intereukin 6 (IL-6)F
R
TTGCCTTCTTGGGACTGATG
CCACGATTTCCCAGAGAACA
55162
Forkhead box transcription factor O1 (FOXO1) F
R
TCCCAATGGCACAGTCCTTA
AGCAGTCCAAAGATGCCCTT
55.2185
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)F
R
AGAACATCATCCCTGCATCC
TCCACCACCCTGTTGCTGTA
60321