Signaling Crosstalk between PPARγ and BMP2 in Mesenchymal Stem Cells

Figure 1

The effect of BMP2 and TRO on adipocyte and osteoblast differentiation of ST2 cells. The scheme of experiments are shown in upper panel. ST2 cells were cultured with one μM troglitazone (TRO) or 50 ng/mL human recombinant BMP2 for seven days. The cells were fixed with 4% formaldehyde/PBS for five min at room temperature, and stained with Oil red O or for alkaline phosphatase (ALPL) activity. Scale bar means 50 μm.