The Nitrated Fatty Acid 10-Nitro-oleate Diminishes Severity of LPS-Induced Acute Lung Injury in Mice
Pulmonary delivery of OA-NO2 reduces LPS-induced lung inflammation. Induction of ALI by i.t. injection of LPS (50 μg) was followed 30 min later by i.t. administration (50 μL) of OA-NO2 (50 μg) or vehicle (10% DMSO). After a further 5.5 h, BAL fluid, plasma, and lung samples were obtained. (a) Total cell and (b) neutrophil number in BAL fluid. Myeloperoxidase activity in (c) lung tissue and (d) BAL fluid. (e) Microscopic examination following staining of BAL fluid. (f) H2O2 production, (g) nitrate concentration, and (h) malonaldehyde/protein ratio in lung. Plasma levels of (i) TNF-, (j) IL-6, (k) KC, and (l) MIP-2. Data are representative of one of two independent experiments with –8 mice per group; ***.