Leptin Antagonizes Peroxisome Proliferator-Activated Receptor-γ Signaling in Growth Plate Chondrocytes
Figure 4
. Leptin signaling is regulated by PPAR activation in growth plate chondrocytes. (a) and (b) Real-time RT-PCR analysis of gene expression of leptin ligand (a) and leptin receptor Ob-Rb (b) in growth plate chondrocytes treated with ciglitazone and/or Ad-PPAR for 5 days. * versus the expression in control cells. (c) Immunoblotting analysis of the levels of total and phosphorylated STAT3 in the lysates of chondrocytes treated with ciglitazone and/or leptin for 5 days. (d) SOCS3 mRNA expression in growth plate chondrocytes treated with ciglitazone and/or Ad-PPAR for 5 days. * versus the expression in control cells.
