Research Article

Time-Qualified Patterns of Variation of PPARγ, DNMT1, and DNMT3B Expression in Pancreatic Cancer Cell Lines

Table 1

Rhythm parameters from fitted single cosinor models for mRNA expression calculated on original values normalized to the first time point of sample collection after serum shock.

BxPC3
PPARGDNMT1DNMT3B

Mesor1.220.941.24
Amplitude0.330.200.16
Acrophase325.32342.87225.6
𝑃 -value0.7800.4300.722

CFPAC
PPARGDNMT1DNMT3B

Mesor1.181.712.42
Amplitude0.400.140.57
Acrophase47.58151.69127.57
𝑃 -value0.4460.8390.753

PANC1
PPARGDNMT1DNMT3B

Mesor1.581.361.65
Amplitude0.620.330.39
Acrophase129.72251.25272.44
𝑃 -value0.0160.0240.630

MIAPACA
PPARGDNMT1DNMT3B

Mesor0.811.211.39
Amplitude0.170.450.14
Acrophase261.86245.4136.43
𝑃 -value0.0100.0670.933

Overall gene expression levels were analyzed for time effect across the timepoints by single cosinor: fit of 24 h cosine to all data by least squares linear regression. Acrophase, the crest time of rhythm, is expressed in degrees. 𝑃 -values refer to test for time effect (zero amplitude).