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PPAR Research
Volume 2012, Article ID 891841, 8 pages
Research Article

Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism

1Programa de Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), 54714 Mexico City, DF, Mexico
2Laboratorio de Rumiología y Metabolismo Nutricional (RuMeN), Facultad de Estudios Superiores Cuautitlán (FES Cuautitlán), UNAM, Bulevar B. Quintana 514-D, Colonia Arboledas, 75230 Querétaro, QRO, Mexico
3Facultad de Medicina Veterinaria y Zootecnia (FMVZ), UNAM and Centro de Enseñanza, Investigación y Extensión en Producción Animal en el Altiplano (CEIEPAA), 76790 Tequisquiapan, QRO, Mexico

Received 23 July 2012; Accepted 27 October 2012

Academic Editor: Juan J. Loor

Copyright © 2012 M. Arévalo-Turrubiarte et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The main adipogenic transcription factor PPAR possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD’s effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 350 kg body weight (BW)) were assigned into 2 treatments: control and 2,4-TZD (8 mg/70 kg BW) and were fed until bulls reached 500 kg BW. They were weighed and their blood was sampled. DNA, RNA, and protein were determined in liver; skeletal muscle; subcutaneous (SC), omental, perirenal adipose tissues (AT) to determine protein synthesis rate and cellular size. Expression of PPAR mRNA was measured in liver and muscle (PPAR , - , and - ) and SC adipose tissue ( ) by real-time PCR. No significant differences were found ( ) in weight gain, days on feed, and carcass quality. Muscle synthesis was greater in controls ( ); cell size was larger with 2,4-TZD ( ). PPAR , - , and - expressions with 2,4-TZD in liver were lower ( ) than in muscle. No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency.