The PPAR Gamma Agonist Troglitazone Regulates Erk 1/2 Phosphorylation via a PPARγ-Independent, MEK-Dependent Pathway in Human Prostate Cancer Cells
Figure 1
Troglitazone inhibits proliferation of PC-3 human prostate cancer cells. (a) PC-3 cells were plated in 6-well plates at 10,000 cells/well and treated for up to six days with DMSO vehicle or troglitazone (2.5–40 μM). Following treatment, the cells in each well were detached using trypsin-EDTA and counted using a Coulter Counter. (b) PC-3 cells were plated in 6 well plates at 10,000 cells/well and treated for up to six days with DMSO vehicle or troglitazone (2.5–40 μM). Following treatment, the cells were pulsed with [3H]-thymidine for 1.5 hours. The level of [3H]-thymidine incorporation was then measured using a scintillation counter. In both panels, each bar represents the mean ± SD for three wells. * compared to DMSO vehicle at each time point. A representative experiment is shown.
