PPAR Research / 2012 / Article / Fig 4

Research Article

PPARα Activation Protects against Anti-Thy1 Nephritis by Suppressing Glomerular NF-κB Signaling

Figure 4

Alteration of mRNA expression of target molecules of transcription factors. mRNAs were obtained from glomeruli of each group of rats. The expression of mRNAs for the target molecules of PPARα (a) and NF-κB (b), including PPARα, ACOX, IκBα, COX2, TNFα, and ICAM1, was measured with real-time PCR. For relative quantification of mRNA, glyceraldehyde-3-phosphate dehydrogenase was used as an internal control, and the relative expression of RNA was calculated by the comparative threshold cycle (Ct) method. The expression was expressed as the change relative to that of the control rats (Fib(−) group of rats at day 0). PCR reactions were carried out in triplicate and averaged for analysis. Values represent means ± SD. Significant differences from the respective day 0 groups are indicated with number signs (# , ## , ### ), while significant differences between regular-diet and clofibrate-diet groups are indicated with asterisks (* , ** , *** ).

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