Structural Features and Transcriptional Activity of Chicken PPARs (, , and )
Figure 3
Effect of serum on the transactivation function of cPPARs. (a), (b). Effects of serum on the transactivation function of PPARs. Serum-induced transactivation function of cPPARs (a) or hPPARs. (b) After transfection with each plasmid mixture, cells were cultured with indicated serum conditions for 36 hrs, then lysed, and luciferase reporter assays were performed as described previously [1]. FBS: fetal bovine serum, CBS: calf bovine serum, BS: adult bovine serum, and ChS: chicken serum. (c) The effect of lipid extract from chicken serum (ChS) on the transactivation function of cPPARs. After being transfected with each plasmid mixture, cells were cultured for 36 hrs in DMEM/10%FBS with or without 5% ChS or ChS lipid extract equivalent of 2% ChS. Then cells were lysed and luciferase assays were performed. Data are derived from triplicate points from two independent experiments and represented as the mean ± SE; .
