Figure 2: ROSI activated the PPARγ response element (PPRE) effectively in GMECs. DMECs were transfected with pGL3-basic-PPRE×3 and pRL-TK vectors. After transfection, cells were treated with different concentration of ROSI. Luciferase and Renilla luciferase assays were performed in triplicate, and the results were expressed relative to the control (0 μmol/L). Luciferase activity data were normalized with Renilla luciferase activity. The data represent mean ± SD of three independent experiments. versus the control group.