Figure 5: Efficacy screening of the two designed shRNA via RT-qPCR and western blot. The efficiency of Ad-sh614 and Ad-sh1006 (transduced with two adenoviruses at 200 multiplicity of infection for 48 h) in decreasing PPARG expression in dairy goat mammary epithelia cells was assessed by RT-qPCR (a) and western blot (b). The data revealed that Ad-sh1006 had the highest knockdown of PPARγ transcript and protein; thus, it was used in the subsequent experiments.